Category: Conferences

The Bush Lab and collaborators will present the following talks and posters at American Society for Mass Spectrometry Annual Conference in San Antonio, TX (June 5-9):

• Evaluating Gas-Phase Folding of Protein Ions Using Cation to Anion Proton Transfer. Kenneth J. Laszlo; Eleanor B. Munger; Stephanie C. Heard; Matthew F Bush. (MOB 8:50)
• Amino Acid Separation using Different Drift Gases in an RF-Confining Drift Cell. Kimberly Davidson; Matthew F Bush (MOC 9:30)
• Analysis of Native-Like Protein and Protein Complex Ions using Structures for Lossless Ion Manipulations (SLIM). Samuel J. Allen; Rachel M. Eaton; Matthew F. Bush (TP 448)

Sam Allen and Matt Bush will also participate in the Fundamentals Interest Group workshop titled “Modification of Commercial Instruments for Fundamental Research”, which will be held in Room 302A on Tuesday, June 7.

We look forward to seeing everyone in San Antonio!

The Bush Lab and collaborators will present the following talks at the 248th American Chemical Society Fall National Meeting, which will be held in San Francisco (August 10-14):

• Droplet sizes, electrospray currents, and nonspecific aggregation in electrokinetically controlled native nanoelectrospray ionization. Kimberly L Davidson, Derek Oberreit, Christopher J Hogan, Matthew F Bush. (Sunday at 3:10 PM in Moscone Center, North Bldg. Room: 130)
• Native electrospray ionization: From initial droplets to final ions. Matthew F. Bush (Monday at 3:20 PM in Moscone Center, North Bldg. Room: 125)
• Determining masses, separating mixtures, and probing structures of native-like ions using selected cation to anion proton transfer (SCAPT) reactions. Matthew F. Bush, Kenneth J. Laszlo. (Wednesday at 3:30 PM in Moscone Center, North Bldg. Room: 125)

Prof. Bush is also organizing the Advances in Ionization Techniques and Mechanisms Symposium, which will be held on Monday in Moscone Center, North Bldg. Room: 125. Prof. Bush thanks the ACS Division of Analytical Chemistry, Agilent, Bruker, Sutter Instrument, Thermo Scientific, and Waters for the generous sponsorship that made this symposium possible. (Symposium Program | 405 kB)

• Introductory Remarks (8:30)
• Development of Multi-Stage Reactive Transmission Mode Desorption Electrospray Ionization Mass Spectrometry for Characterizing Multicatalytic Reactions. Richard H. Perry, Kevin C. Peters, Kevin E. Parker, Troy J. Comi (8:35)
• Development of Solvent-Free Ambient Mass Spectrometry for Green Chemistry Applications. Hao Chen, Pengyuan Liu, Amanda Forni. (9:05)
• Ambient ionization mass spectrometric imaging with high spatiotemporal resolution. Richard N. Zare, Jae Kyoo Lee, Hong Gil Nam (9:35)
• Intermission (10:05)
• Development of a surface acoustic wave nebulization source for mass spectrometry. David Goodlett, Tao Liang, Scott R Heron, Shivangi Awasthi, Sung H Yoon, Yue Huang, Michael Wilson, David P A Kilgour. (10:05)
• Magic Matrices for Ionization in Mass Spectrometry. Sarah Trimpin. (10:55)
• Low zeptomole detection limits with capillary electrophoresis coupled to an improved nanospray. Norman J. Dovichi, Liangliang Sun, Guijie Zhu (11:25)
• Break for Lunch (11:55)
• Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI): From Fundamentals to Tissue and Chemical Imaging. David C. Muddiman. (1:30)
• Matrix-free, ambient pressure mass spectrometry via ultra-intense laser vaporization. Robert J. Levis. (2:00)
• Molecular modeling of the ejection mechanisms of macromolecules from charged nanodroplets. Styliani Consta. (2:30)
• Intermission. (3:00)
• Native Electrospray Ionization: From Initial Droplets to Final Ions. Matthew F. Bush. (3:20)
• Charges in protein electrospray ionization: Like or opposite? Rachel R. Ogorzalek Loo, Joseph A. Loo. (3:50)
• Supercharging, Fast Mixing and Charge Detection in Mass Spectrometry. Evan R. Williams. (4:20)

Kim Davidson is also co-organizing the Graduate Student Symposium on International Collaborations with International Impact: Chemistry for Global Change, which will be held on Tuesday in the Esplanade Ballroom 304 in the south building of the Moscone Center.

We look forward to seeing everyone in San Francisco!

Erin and I hope that everyone enjoyed this year’s workshop! Mike Bowers, Colin Creaser, and Herb Hill have kindly agreed to share their slides (links below).

Applying Ion Mobility to Biological Problems
Erin Baker (PNNL) and Matt Bush (University of Washington), presiding
5:45 PM on Monday, June 16th. Room 314-317.

Results from ion mobility mass spectrometry studies are increasingly used to answer questions in biology, including applications to metabolomics, proteomics, targeted interactions, and large molecules. This is in part attributed to the increasing performance and selection of commercial ion mobility mass spectrometry instrumentation, which has made it easier to integrate ion mobility technologies into mass spectrometry workflows. In this workshop, 3 members of the community will present their perspectives on the future of ion mobility MS in biology. These perspectives will be followed by a panel discussion of the challenges that arise in different types of ion mobility studies, what is possible today, and opportunities for the future.

Perspectives on the Future of Ion Mobility MS

• Michael Bowers (University of California, Santa Barbara) (Slides | PDF | 2.3 mb)
• Colin Creaser (Loughborough University) (Slides | PDF | 220 kb)
• Herb Hill (Washington State University) (Slides | PDF | 5.9 mb)

Panel Discussion

• Michael Bowers (University of California, Santa Barbara)
• Colin Creaser (Loughborough University)
• Kevin Giles (Waters)
• Michael Grössl (TOFWERKS)
• Herb Hill (Washington State University)
• Mel Park (Bruker)
• Brad Schneider (AB SCIEX)
• George Stafford (Agilent)
• Danielle Toutoungi (Owlstone)

Please send questions or suggestions for the panel discussion to Erin Baker (Erin.Baker@pnnl.gov) or Matt Bush (mattbush@uw.edu). We look forward to seeing everyone in Baltimore!

The Bush Lab and collaborators will present the following talks and posters at American Society for Mass Spectrometry Annual Conference in Baltimore, MD (June 15-19):

• Determining Masses, Separating Mixtures, and Probing Structures of Native-Like Ions Using Selected Cation to Anion Proton Transfer (SCAPT) Reactions. Laszlo, K. J.; Bush, M. F.  (MOD 9:30-9:50)
• Supercharging of Native Protein Complexes: Effects of Polarity and Evidence for Multiple Mechanisms. Allen, S. J.; Stachl, C. N.; Bush, M. F. (MOC 3:30-3:50)
• Supercharging of Native-Like Proteins and Protein Complexes: Effects of m-Nitrobenzyl Alcohol versus Sulfolane. Stachl, C. N.; Allen, S. J.; Bush, M. F. (WP669)
• Probing Protein-Protein and Protein-DNA Interactions by Native Mass Spectrometry and Global Hydrogen Deuterium Exchange using Surface Acoustic Wave Nebulization (SAWN). Monkkonen, L.; Heron, S.; Bush, M. F.; Catalano, C. E.; Goodlett, D. R. (ThP93)
• Additional Models of a Mammalian Cryptochrome – E3 Ubiquitin Ligase Complex Restrained by Results from Ion Mobility Mass Spectrometry. Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. (ThP470)
• Droplet sizes, electrospray currents, and nonspecific aggregation in electrokinetically controlled native nanoelectrospray ionization. Davidson, K. L.; Oberreit, D. R.; Hogan, C. J.; Bush, M. F. (ThOC 4:10-4:30)

Matt Bush and Erin Baker (PNNL) are also organizing the “Applying Ion Mobility to Biological Problems Workshop”, which is organized by the Ion Mobility MS Interest Group. The workshop will be held on Monday from 5:45-7:00 pm in Rooms 314-317. Click here for more details.

We look forward to seeing everyone in Baltimore!

The Bush Lab and collaborators are presenting the following talks at the Cascadia Proteomics Symposium, which takes place at the Institute for Systems Biology in Seattle from July 15-16.

• Ion Mobility Mass Spectrometry of Native Protein Complex Anions Samuel J. Allen, Alicia M. Schwartz, Matthew F. Bush (Tuesday @ 10:15 AM)
  
• Selected Cation to Anion Proton Transfer Kenneth J. Laszlo, Matthew F. Bush (Tuesday @ 10:50 AM)
• Assembly of the Type II Secretion ATPase Probed by Native Mass Spectrometry Samuel T. Marionni, Connie Lu, Stewart Turley, Young-Jun Park, Kelly Lee, Marcella Patrick, Ripal Shah, Maria Sandkvist, Wim G.J. Hol, Matthew F. Bush (Tuesday @ 1:00 PM)

• Prof. Bush will present a talk at the Yale Biological Mass Spectrometry Symposium on June 25th. MFB thanks Prof. Andrew Philips and Suzanne Decatur for organizing this symposium.
• Prof. Bush will present the University of Minnesota Particle Technology Seminar  on June 20th. MFB thanks Prof. Chris Hogan for hosting this visit.
• Ion Mobility Mass Spectrometry of Small Molecule, Polymer, and Native Protein Complex Anions Samuel J. Allen; Alicia M. Schwartz; Matthew F. Bush. American Society for Mass Spectrometry Conference. Wednesday, June 12, Poster #737.

Ion Mobility Mass Spectrometry: New Instrumentation & Enabling Technologies
Matthew F. Bush presiding
Tuesday, June 11th at 5:45 PM | Room 205 AB

The performance of conventional and differential ion mobility mass spectrometers has escalated dramatically in recent years, which can be attributed to improved separations, more efficient ion transfer, and tighter integration of ion mobility devices into hybrid instruments. These improvements enable intricate and sensitive experiments probing mobility selected or separated ions, which has led to the adoption of ion mobility technologies in a wide range of applications, including challenges in gas-phase ion structure determination, native mass spectrometry, proteomics, and systems biology. We will showcase the latest developments in ion mobility mass spectrometry instrumentation and discus remaining challenges.

Prof. Stephen Valentine (West Virginia University)
Miniaturization of Overtone Ion Mobility Spectrometry

Dr. Guillermo Vidal-de-Miguel (SEADM)
Developments in Transversal Modulation IMS (TMIMS): an Add-on enabling absolute IMS-IMS pre-filtration in tandem with MS, and providing 100% DC

Dr. Dimitris Papanastasiou (Fasmatech)
Implementation of a novel Differential Mobility Spectrometry Platform in the Fore Vacuum Region of a Mass Spectrometer

Dr. Melvin Park (Bruker)
High Resolution Trapped Ion Mobility Spectrometry and the Beginnings of a Theory

Dr. Yehia Ibrahim (Pacific Northwest National Laboratory)
The development of the PNNL IMS platform: challenges and solutions