Ken Laszlo

 

ken_webpage

Contact: klaszlo@uw.edu

Bio

Ken Laszlo graduated from the Georgia Institute of Technology in spring 2012 with a BS in chemistry. While there he participated in undergraduate research with Dr. Facundo Fernández investigating cyclodextrins as mobility shift reagents in ion mobility – mass spectrometry (IM-MS).

Ken’s research with Prof. Bush has consisted of ion mobility, electron transfer dissociation (ETD), and Cation-to-Anion Proton Transfer Reactions (CAPTR), and his future interest lies in developing new analysis and bioanalytical tools to characterize biomolecule and biotherapeutics.

Research

  • Cation to Anion Proton Transfer Reactions (CAPTR): Ken initiated several projects involving gas-phase proton transfer from multiply charged cation native-like protein ions to anions. We are exploring this technology for analytical purposes, as well as to study the gas-phase folding of denatured proteins. The latter set of experiments are being used to study the relationship between protein structure and gas-phase charge state.
  • Electron Transfer Dissociation Mechanisms: In collaboration with the computational data provided by the TureÄŤek group, Ken investigated the structure of c and z fragment ions. Our research has shown that fragmentation pathways are dependent on reactive intermediate structures following electron transfer.

Publications

9.    Laszlo, Kenneth J., Eleanor B. Munger, and Matthew F Bush. “Effects of Solution Structure on the Folding of Lysozyme Ions in the Gas Phase.” The Journal of Physical Chemistry B, 2017, doi:10.1021/acs.jpcb.7b00783. (Link|PUBMED)

8.    Laszlo, Kenneth J., John H. Buckner, Eleanor B. Munger, and Matthew F. Bush. “Native-Like and Denatured Cytochrome c Ions Yield Cation-to-Anion Proton Transfer Reaction Products with Similar Collision Cross-Sections.” Journal of The American Society for Mass Spectrometry, 2017, doi:10.1007/s13361-017-1620-4. (Link)

7.    Pepin, Robert, Alessio Petrone, Kenneth J. Laszlo, Matthew F. Bush, Xiaosong Li, and František TureÄŤek. “Does Thermal Breathing Affect Collision Cross Sections of Gas-Phase Peptide Ions? An Ab Initio Molecular Dynamics Study.” The Journal of Physical Chemistry Letters, July 8, 2016, 2765–71. doi:10.1021/acs.jpclett.6b01187. (Link)

6.    Laszlo, Kenneth J., Eleanor B. Munger, and Matthew F. Bush. “Folding of Protein Ions in the Gas Phase after Cation-to-Anion Proton-Transfer Reactions.” Journal of the American Chemical Society, 2016, 138, 9581–88. doi:10.1021/jacs.6b04282.  (Link)

5.    Pepin, Robert, Kenneth J. Laszlo, Aleš Marek, Bo Peng, Matthew F. Bush, Helène Lavanant, Carlos Afonso, and František TureÄŤek. “Toward a Rational Design of Highly Folded Peptide Cation Conformations. 3D Gas-Phase Ion Structures and Ion Mobility Characterization.” Journal of The American Society for Mass Spectrometry, July 11, 2016, 1–14. doi:10.1007/s13361-016-1437-6. (Link)

4.    Laszlo, Kenneth J. , and Matthew F. Bush. “Analysis of Native-Like Proteins and Protein Complexes Using Cation to Anion Proton Transfer Reactions (CAPTR).” Journal of The American Society for Mass Spectrometry, 2015, 26 2152–61. doi:10.1007/s13361-015-1245-4. (Link)

3.    Marek, Aleš, Christopher J. Shaffer, Robert Pepin, KristĂ­na Slováková, Kenneth J. Laszlo, Matthew F. Bush, František TureÄŤek. “Electron Transfer Reduction of the Diazirine Ring in Gas-Phase Peptide Ions. On the Peculiar Loss of [NH4O] from Photoleucine.” JASMS, 2014, 26, 415-431  (Link|PUBMED)

2.    Pepin, Robert, Kenneth J. Laszlo, Bo Peng, Aleš Marek, Matthew F. Bush, and František TureÄŤek. “Comprehensive Analysis of Gly-Leu-Gly-Gly-Lys Peptide Dication Structures and Cation-Radical Dissociations Following Electron Transfer: From Electron Attachment to Backbone Cleavage, Ion–Molecule Complexes, and Fragment Separation.” J. Phys. Chem. A, 2014, 118, 308–324. (Link|PUBMED)

1.    Marek, Aleš, Robert Pepin, Bo Peng, Kenneth J. Laszlo, Matthew F. Bush, and František TureÄŤek. “Electron Transfer Dissociation of Photolabeled Peptides. Backbone Cleavages Compete with Diazirine Ring Rearrangements.” Am. Soc. Mass Spectrom,. 2013, 24, 1641–1653. (Link|PUBMED)

 

Oral Presentations

    10.    Laszlo, Kenneth J.; Munger, E.B.; Buckner, J.H.; Heard, S.C.; Bush, Matthew F. “Foling Proteins in the Gas-Phase” Georgia Institute of Technology, Atlanta, GA, 03/06/2017

    9.    Laszlo, Kenneth J.; Munger, E.B.; Heard, S.C.; Bush, Matthew F. “Effects of Charge State on the Structures of Denatured Protein Ions” Cascadia Proteomics Symposium, Seattle, WA, 7/13/2016

    8.    Laszlo, Kenneth J.; Munger, E.B.; Heard, S.C.; Bush, Matthew F. “Evaluating Gas-Phase Folding of Protein Ions Using Cation to Anion Proton Transfer Reactions” 64nd ASMS Conference on Mass Spectrometry and Allied Topics, San Antonio, TX, 6/6/2016

    7.    Laszlo, Kenneth J.; Munger, E.B.; Bush, Matthew F. “Using Cation to Anion Proton Transfer Reactions to Probe the Relationship Between Charge and Structure for Protein Ions” 2016 Conference on Ion Chemistry and Mass Spectrometry, Lake Arrowhead, CA, 1/17/2016

    6.    Laszlo, Kenneth J.; Bush, Matthew F. “Cation to Anion Proton Transfer Reactions of Protein Complexes: Effects of Charge on Structure” ASMS Asilomar Conference on Native Mass Spectrometry-Based Structural Biology, Pacific Grove, CA, 10/20/2015

    5.    Laszlo, K. J.; Munger, E.B.; Bush, M.F. “On the Folding of Denatured Proteins in the Gas Phase”63rd ASMS Conference on Mass Spectrometry and Allied Topics, Workshop on Gas-Phase Ion Chemistry: Thermodynamics, Kinetics, Structures and Spectroscopy Fundamentals Interest Group, St. Louis, MO, 6/3/2015

    4.    Laszlo, K. J.; Munger, E.B.; Bush, M.F. “Effects of charge state on the structures of protein ions in the gas phase: Results from ion/ion proton transfer reactions”12th Uppsala Conference on Electron Capture and Transfer Dissociation, Lake Arrowhead, CA, 3/25/2015

3.    Laszlo, K. J.; Munger, E.B.; Bush, M.F. “Cation to Anion Proton Transfer Reactions (CAPTR) of Protein Ions: Analytical Utility and Effects of Charge State on Ion Structure”2015 Conference on Ion Chemistry and Mass Spectrometry, Lake Arrowhead, CA, 1/17/2015

2.    Laszlo, K. J.; Bush, M.F. “Determining Masses, Separating Mixtures, and Probing Structures of Native-Like Ions using Selected Cation to Anion Proton Transfer (SCAPT)” 62nd ASMS Conference on Mass           Spectrometry and Allied Topics, Baltimore, MD, 6/16/2014

1.    Laszlo, K. J.; Bush, M.F. “Selected Cation to Anion Proton Transfer” Cascadia Proteomics Symposium, Seattle, WA, 7/16/2013

 

Poster Presentations

    8.    Laszlo, Kenneth J.; Munger, E.B.; Heard, S.C.; Bush, Matthew F. “Effects of Charge State on the Structures of Denatured Protein Ions” Cascadia Proteomics Symposium, Seattle, WA, 7/13/2016

    7.    Laszlo, Kenneth J.; Bush, Matthew F. “Analysis of Native-Like Proteins and Protein Complexes Using Cation to Anion Proton Transfer Reactions (CAPTR)” 2016 Center for Process Analysis and Control (CPAC) Spring Meeting, Seattle, WA, 5/2/2016

    6.    Laszlo, Kenneth J.; Bush, Matthew F. “Cation to Anion Proton Transfer Reactions of Protein Complexes: Effects of Charge on Structure” ASMS Asilomar Conference on Native Mass Spectrometry-Based Structural Biology, Pacific Grove, CA, 10/19-20/2015

5.    Laszlo, Kenneth J.; Munger, E.B., Bush, Matthew F. “Effects of Charge State on the Structures of Protein Ions: Results from Cation to Anion Proton Transfer Reactions” 63rd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, 6/3/2015

    4.    Yen, Gloria; Laszlo, Kenneth J.; Huang, Yue; Heron, Scott; Bush, Matthew F.; Goodlett, David; Nilsson, Erik. “Effects of Charge State on the Structures of Protein Ions: Results from Cation to Anion Proton Transfer Reactions” 63rd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, 6/3/2015

    3.    Laszlo, Kenneth J.; Bush, Matthew F. “Selected Cation to Anion Proton Transfer” Cascadia Proteomics Symposium, Seattle, WA 7/16/13

    2.    Laszlo, Kenneth J.; Dwivedi, Prabha; FernandĂ©z, Facundo M., “Ion Mobility Mass Spectrometric Study of Gas-Phase Cyclodextrin-Lipid Inclusion Complexes” American Society for Mass Spectrometry Annual Conference, Vancouver, BC 5/22/12

    1.    Laszlo, Kenneth J.; FernandĂ©z, Facundo M., “Cyclodextrins as a Mobility Shift Reagent for the Analysis of Prebiotic Mixtures”, Annual Conference for the Center for Chemical Evolution, Atlanta, GA 8/4/2011

 

Awards

10.    2016-17 Alma Mater Travel Award (~3-4 awarded annually, issued by the University of Washington Department of Chemistry)

9.    2014-15 Graduate Student Merit Fellowship (~5-10 annually, issued by the University of Washington Department of Chemistry)

8.    Molecular Biophysics Training Grant (MBTG) recipient (2-4 awards annually within biophysical sciences at UW)

7.    Richard A. Schaeffer Memorial Travel Award (62nd Annual American Society of Mass Spectrometry Conference on Mass Spectrometry and Applied Topics, June 2015, ~four awards annually)

6.    ARCS Fellowship, Seattle Chapter (2012-2015, ~35 awards annually across WA)

5.    ACS Award in Analytical Chemistry (2012, one awarded per university)

4.    Outstanding 1-to-1 Tutor (2012)

3.    Presidents Undergraduate Research Award (Spring 2011)

2.    Zell Miller Scholar (2011-2012)

1.    HOPE Scholar (2008-2011)