Out of the Attic, Into the Lab: building a shrink ray is harder than it looks

On August 1st, Rae Eaton, a graduate student in the Bush Lab, presented a talk entitled “Out of the Attic, Into the Lab: building a shrink ray is harder than it looks” The talk was part of the Science and a Movie program, a series put on by Central Cinema and the Pacific Science Center. The series pairs talks from scientists in the Seattle area with silly or classic movies.

In the talk, Rae used stories from the world of bioanalytical chemistry to discuss the challenges home inventors can run into working in unusual spaces, and explores the benefits a lab offers versus working by oneself in their garage. The talk was followed by a screening of “Honey I Shrunk the Kids”.

Thanks to Central Cinema and the Pacific Science Center’s Meet a Scientist program for the opportunity!

New NSF Award!

The Bush Lab was just awarded a grant from the National Science Foundation for a project titled “Bridging the Gap Between Observables from Ion Mobility Mass Spectrometry and the Structures of Native Proteins” (1807382). We are excited to pursue this research and are grateful for this financial support from the NSF.

Bush Lab at ASMS 2018

Members of the Bush Lab will present the following talks at American Society for Mass Spectrometry Annual Conference in San Diego (June 3 – 7, 2018):

  • Ion Mobility of Proteins in Nitrogen Gas: Effects of Charge State, Charge Distribution, and Structure Characterized using Trajectory Method Calculations. Daniele Canzani; Matthew F, Bush. (MP 407)
  • Collision-Induced Unfolding and Dissociation Reveal the Location of Ni(II) Binding in the Dimer of the Alpha-Crystallin Domain of HSPB5. Seoyeon Hong; Matthew F. Bush. (MP 745)
  • Memory of the Condensed-Phase in the Gas-Phase: Effects of Solution, Charge, and Energy on Structures of Serum Albumin IonsMeagan Gadzuk-Shea; Matthew F Bush. (WOB 10:10)

We look forward to seeing everyone in San Diego!

New Publication: Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas

Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas: improving the accuracy of trajectory method calculations. Jong Wha Lee, Hyun Hee L. Lee, Kimberly L. Davidson, Matthew F. Bush, Hugh I. Kim. Analyst 2018, in press. (Link)

The investigation of ion structures based on a combination of ion mobility mass spectrometry (IM-MS) experiments and theoretical collision cross section (CCS) calculations has become important to many fields of research. However, the accuracy of current CCS calculations for ions in nitrogen drift gas limits the information content of many experiments. In particular, few studies have evaluated and attempted to improve the theoretical tools for CCS calculation in nitrogen drift gas. In this study, Continue reading “New Publication: Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas”

New Publication: Collision Cross Sections and Ion Structures

Collision Cross Sections and Ion Structures: Development of a General Calculation Method via High-quality Ion Mobility Measurements and Theoretical Modeling. Jong Wha Lee, Kimberly L. Davidson, Matthew F. Bush, Hugh I. Kim. Analyst 2017, in press. (Link)

Ion mobility mass spectrometry (IM-MS) has become an important tool for the structural investigation of ions in the gas phase. Accurate theoretical evaluation of ion collision cross sections (CCSs) is essential for the effective application of IM-MS in structural studies. However, current theoretical tools have limitations in accurately describing a broad range of ions from small molecules to macromolecules. Continue reading “New Publication: Collision Cross Sections and Ion Structures”