Author: Matt Bush

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Upcoming Presentations: June

  • Prof. Bush will present a talk at the Yale Biological Mass Spectrometry Symposium on June 25th. MFB thanks Prof. Andrew Philips and Suzanne Decatur for organizing this symposium.
  • Prof. Bush will present the University of Minnesota Particle Technology Seminar  on June 20th. MFB thanks Prof. Chris Hogan for hosting this visit.
  • Ion Mobility Mass Spectrometry of Small Molecule, Polymer, and Native Protein Complex Anions Samuel J. Allen; Alicia M. Schwartz; Matthew F. Bush. American Society for Mass Spectrometry Conference. Wednesday, June 12, Poster #737.
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Ion Mobility MS Workshop Program

Ion Mobility Mass Spectrometry: New Instrumentation & Enabling TechnologiesASMS
Matthew F. Bush presiding
Tuesday, June 11th at 5:45 PM | Room 205 AB

The performance of conventional and differential ion mobility mass spectrometers has escalated dramatically in recent years, which can be attributed to improved separations, more efficient ion transfer, and tighter integration of ion mobility devices into hybrid instruments. These improvements enable intricate and sensitive experiments probing mobility selected or separated ions, which has led to the adoption of ion mobility technologies in a wide range of applications, including challenges in gas-phase ion structure determination, native mass spectrometry, proteomics, and systems biology. We will showcase the latest developments in ion mobility mass spectrometry instrumentation and discus remaining challenges.

Prof. Stephen Valentine (West Virginia University)
Miniaturization of Overtone Ion Mobility Spectrometry

Dr. Guillermo Vidal-de-Miguel (SEADM)
Developments in Transversal Modulation IMS (TMIMS): an Add-on enabling absolute IMS-IMS pre-filtration in tandem with MS, and providing 100% DC

Dr. Dimitris Papanastasiou (Fasmatech)
Implementation of a novel Differential Mobility Spectrometry Platform in the Fore Vacuum Region of a Mass Spectrometer

Dr. Melvin Park (Bruker)
High Resolution Trapped Ion Mobility Spectrometry and the Beginnings of a Theory

Dr. Yehia Ibrahim (Pacific Northwest National Laboratory)
The development of the PNNL IMS platform: challenges and solutions

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New Publication: SCFFBXL3 ubiquitin ligase targets cryptochromes at their cofactor pocket

cry2The cryptochrome (CRY) flavoproteins act as blue-light receptors in plants and insects, but perform light-independent functions at the core of the mammalian circadian clock. To drive clock oscillations, mammalian CRYs associate with the Period proteins (PERs) and together inhibit the transcription of their own genes. The SCFFBXL3 ubiquitin ligase complex controls this negative feedback loop by promoting CRY ubiquitination and degradation. However, the molecular mechanisms of their interactions and the functional role of flavin adenine dinucleotide (FAD) binding in CRYs remain poorly understood. Here we report crystal structures of mammalian CRY2 in its apo, FAD-bound and FBXL3–SKP1-complexed forms. Distinct from other cryptochromes of known structures, mammalian CRY2 binds FAD dynamically with an open cofactor pocket. Notably, the F-box protein FBXL3 captures CRY2 by simultaneously occupying its FAD-binding pocket with a conserved carboxy-terminal tail and burying its PER-binding interface. This novel F-box-protein–substrate bipartite interaction is susceptible to disruption by both FAD and PERs, suggesting a new avenue for pharmacological targeting of the complex and a multifaceted regulatory mechanism of CRY ubiquitination.

SCFFBXL3 ubiquitin ligase targets cryptochromes at their cofactor pocket Weiman Xing, Luca Busino, Thomas R. Hinds, Samuel T. Marionni, Nabiha H. Saifee, Matthew F. Bush, Michele Pagano, Ning Zheng. Nature 2013, 496, 64–68. (Link|PUBMED)

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Ion Mobility MS Workshop @ ASMS 2013

ASMS

Every year, the Ion Mobility MS Interest Group organizes a workshop that is held during the American Society for Mass Spectrometry Annual Meeting. Preliminary information for this year’s workshop is outlined below. Please contact Matt Bush with any suggested topics, questions, or speakers for this workshop.

Ion Mobility MS: New Instrumentation & Enabling Technologies

Tuesday, June 11, from 5:45 – 7:00 PM (Minneapolis Convention Center, Room TBD)

The performance of conventional and differential ion mobility mass spectrometers has escalated dramatically in recent years, which can be attributed to improved separations, more efficient ion transfer, and tighter integration of ion mobility devices into hybrid instruments. These improvements enable intricate and sensitive experiments probing mobility selected or separated ions, which has led to the adoption of ion mobility technologies in a wide range of applications, including challenges in gas-phase ion structure determination, native mass spectrometry, proteomics, and systems biology. We will showcase the latest developments in ion mobility mass spectrometry instrumentation and discus remaining challenges.