Author: Matt Bush

Congratulations to Dr. Lindsey Ulmer, who successfully defended her thesis titled “New Photo-Crosslinking Mass Spectrometry Approaches for the Study of Intrinsically Disordered Proteins.” Lindsey has accepted an offer to join Waters Corporation as Senior Scientist for the Evaluations group in the Cell and Gene Consumables team. We all wish Lindsey great success and happiness in Boston!

Congratulations to Lindsey Ulmer, whose research was just published in the Journal of Proteome Research! This research demonstrates significant advances in identifying residue-specific crosslinks originating from a photoreactive amino acid, which we have been using to characterize proteins with intrinsic disorder.

High-Performance Workflow for Identifying Site-Specific Crosslinks Originating from a Genetically Incorporated, Photoreactive Amino Acid. Lindsey D. Ulmer, Daniele Canzani, Christopher N. Woods, Natalie L. Stone, Maria K. Janowska, Rachel E. Klevit, Matthew F. Bush. J. Proteome Res. 2024, 23, 3560–3570. (Link)

Congratulations to May Constabel, who successfully defended her thesis titled “Programmed Temperature Electrospray Ionization (ptESI) for Thermal Cycling of Proteins”. May will soon complete her degree in the M.S. in Applied Chemical Science and Technology (MSACST) program. May has accepted an offer to join the laboratory of Prof. Anouk Rijs at the Vrije Universiteit Amsterdam as a PhD student. We all wish May great success and happiness in the Netherlands!

Congratulations to Theresa Gozzo, whose research was just published in Analytical Chemistry! This research demonstrates the synergy between cation-to-anion proton-transfer reactions (CAPTR), energy-dependent IM-MS, and similarity scoring for the analysis of biotherapeutics.

Quantitatively Differentiating Antibodies Using Charge-State Manipulation, Collisional Activation, and Ion Mobility – Mass Spectrometry. Theresa A. Gozzo, Matthew F. Bush. Anal. Chem. 2023, , DOI: 10.1021/acs.analchem.3c04638. (Link)

Congratulations to Theresa Gozzo, who just successfully defended her dissertation!

The Bush Lab enjoyed this year’s PacMass members night! PacMass is the Pacific Northwest Mass Spectrometry Group, and brings together mass spectrometry enthusiasts from the region, including those from the University of Washington, independent research organization, industry, and government. In addition to quarterly research talks, we get together each November to present posters and socialize. Here are a few of our posters.

Congratulations to Ben Zercher and Bruce Feng, whose research was just published in the International Journal of Mass Spectrometry! This research contributes to our long-term goal of developing high-performance, multi-dimensional ion mobility experiments that use electrostatic drift fields.

Until December 10, 2023, this article can be downloaded without a subscription from the following link: https://authors.elsevier.com/a/1hyt34xT2vCyVD.

Towards IMⁿ with Electrostatic Drift Fields: Resetting the Potential of Trapped Ions Between Dimensions of Tandem Ion Mobility. Benjamin P. Zercher, Yuan Feng, Matthew F. Bush. Int. J. Mass Spectrom. 2023, 495, 117163. (Link)

Native mass spectrometry studies often report initial pH, but is that the pH of samples during experiments? Using ratiometric fluorescence imaging, we find that sample pH can drift during experiments. Congratulations to Meagan Gadzuk-Shea, Evan Hubbard, and Theresa Gozzo, whose paper was just published in the Journal of the American Society for Mass Spectrometry!

Sample pH Can Drift during Native Mass Spectrometry Experiments: Results from Ratiometric Fluorescence Imaging. Meagan M. Gadzuk-Shea, Evan E. Hubbard, Theresa A. Gozzo, Matthew F. Bush. J. Am. Soc. Mass Spectrom. 2023, 34, 1675–1684. (Link)

To what extent and on what timescales do the structures of proteins change in the gas phase? We used time-dependent IM-IM-MS and collision-induced unfolding to find out. Congratulations to Ben Zercher, Cece Hong, Addison Roush, and Bruce Feng, whose paper was just published in Analytical Chemistry.

This article was selected for ACS Editors’ Choice, which is a collection designed to feature scientific articles of broad public interest that are made free to readers. One selection is made per day, across all 75+ ACS journals.

Are the Gas-Phase Structures of Molecular Elephants Enduring or Ephemeral? Results from Time-Dependent, Tandem Ion Mobility. Benjamin P. Zercher, Seoyeon Hong, Addison E. Roush, Yuan Feng, Matthew F. Bush. Anal. Chem. 2023, 95, 9589–9597. (Link)

It was great to catch up with Meagan Gadzuk-Shea (now a Senior Scientist at Astra Zeneca), Daniele Canzani (now a Senior Scientist at Talus Biosciences), and Evan Hubbard (now in laboratory of Ryan Julian at UC Riverside) at the American Society for Mass Spectrometry conference in Houston, TX.