Sam Marionni

Contact: LinkedIn

Bio

• Sam Marionni came to the University of Washington shortly after receiving his B.S. in chemistry from the University of Maryland in 2009. As an undergraduate, Sam worked in the research group of Professor T. Ashton Cropp, investigating the biosynthetic pathway of piericidin in Streptomyces mobaraensis. While at Maryland, Sam held a Howard Hughes Undergraduate Research Fellowship for two years and in his final year received the Greater Washington Institute of Chemists Award. Sam spent his first two years at UW in the research group of Professor Gojko Lalic studying transition metal-catalyzed cross-coupling reactions, resulting in the successful development of a stereoselective, copper-catalyzed synthesis of allenes. In the summer of 2011, Sam joined the Bush group, where he uses ion mobility and native mass spectrometry techniques to investigate the structures of large protein complexes.

Awards

• Klaus and Mary Ann Saegebarth Endowed Fellowship in Chemistry
• Greater Washington Institute of Chemists Award
• Howard Hughes Undergraduate Research Fellowship

Publications (h-index: 4 | Google Scholar)

• Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity Connie Lu, Stewart Turley, Samuel T. Marionni, Young-Jun Park, Kelly K. Lee, Marcella Patrick, Ripal Shah, Maria Sandkvist, Matthew F. Bush, Wim G.J. Hol. Structure, 2013, 21, 1707–1717.
• SCF^FBXL3 ubiquitin ligase targets cryptochromes at their cofactor pocket Weiman Xing, Luca Busino, Thomas R. Hinds, Samuel T. Marionni, Nabiha H. Saifee, Matthew F. Bush, Michele Pagano, Ning Zheng. Nature 2013, 496, 64–68.
• Asymmetric Synthesis of Trisubstituted Allenes: Copper-Catalyzed Alkylation and Arylation of Propargylic Phosphates Mycah R. Uehling, Samuel T. Marionni, Gojko Lalic. Org. Lett.2012, 14, 362-365.
• Site-Specific Incorporation of Fluorotyrosines into Proteins in Escherichia coli by Photochemical Disguise Bryan J. Wilkins, Samuel Marionni, Douglas D. Young, Jia Liu, Yan Wang, Martino L. Di Salvo, Alexander Deiters and T. Ashton Cropp. Biochemistry, 2010, 49, 1557-1559.

Oral Presentations

• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Native Ion Mobility Mass Spectrometry Results as Constraints for Structural Modeling of Conformationally Heterogeneous Complexes” Quarterly UW Proteomics Resource Seminar, Seattle, WA, 5/26/2015.
• Marionni, S. T.; Lu, C.; Turley, S.; Park, Y.-J.; Lee, K. K.; Patrick, M.; Shah, R.; Sandkvist, M.; Hol, W. G. J.; Bush, M. F. “Assembly of the Type II Secretion ATPase Probed by Native Mass Spectrometry” Cascadia Proteomics Symposium, Seattle, WA, 7/16/2013.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Ion Mobility Mass Spectrometry of a Circadian Clock Protein Complex Reveals a Ligand-Dependent Conformational Switch” Cascadia Proteomics Symposium, Seattle, WA, 7/19/2012.

Poster Presentations

• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Additional Models of a Mammalian Cryptochrome – E3 Ubiquitin Ligase Complex Restrained by Results from Ion Mobility Mass Spectrometry” PacMass Members’ Night, Seattle, WA, 11/19/14.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Additional Models of a Mammalian Cryptochrome – E3 Ubiquitin Ligase Complex Restrained by Results from Ion Mobility Mass Spectrometry” 62nd American Society for Mass Spectrometry Conference, Baltimore, MD, 6/16/14
• Marionni, S. T.; Lu, C.; Turley, S.; Park, Y.-J.; Lee, K. K.; Patrick, M.; Shah, R.; Sandkvist, M.; Hol, W. G. J.; Bush, M. F. “Assembly of the Type II Secretion ATPase Probed by Native Mass Spectrometry” Cascadia Proteomics Symposium, Seattle, WA, 7/16/13.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Ion Mobility Mass Spectrometry of a Circadian Clock Protein Complex Reveals a Ligand-Dependent Conformational Switch” PACMASS Members’ Evening, Seattle, WA, 10/29/12.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Ion Mobility Mass Spectrometry of a Circadian Clock Protein Complex Reveals a Ligand-Dependent Conformational Switch” Cascadia Proteomics Symposium, Seattle, WA, 7/19/12.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Ion Mobility Mass Spectrometry of a Circadian Clock Protein Complex Reveals a Ligand-Dependent Conformational Switch” Seattle Ubiquitin Research Group Poster Session, Seattle, WA, 7/12/12.
• Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. “Ion Mobility Mass Spectrometry of a Circadian Clock Protein Complex Reveals a Ligand-Dependent Conformational Switch” 60th American Society for Mass Spectrometry Conference, Vancouver, BC, 5/23/12.
• Marionni, S. T.;* Allen, S. J.;* Bush, M. F. “Gas-Phase Structural Biology of Proteins and their Complexes” Biological Physics, Structure and Design Retreat, Seattle, WA, 11/28/11.
• Marionni, S. T.;* Allen, S. J.;* Bush, M. F. “Gas-Phase Structural Biology of Proteins and their Complexes” PacMass Members’ Night, Seattle, WA, 11/8/11.